Mmp10 is necessary for <i>Kras^LA2</i>-induced lung tumorigenesis <i>in vivo.</i>

<p><b>A</b>) Immunohistochemical staining of <i>Kras^LA2</i> lung tumor for mouse MMP10. Higher magnification image of Mmp10 immunostaining is shown in the inset. Quantitative analysis of <b>B</b>) tumor number, <b>C</b>) tumor size and <b>D)</b> tumor burden in <i>Kras^LA2</i> and <i>Kras^LA2/Mmp10^−/−</i> mice. Columns, mean; bars, SEM, n = 13, (*) denotes p = 0.04. E) Tumors from <i>Kras^LA2</i> and <i>Kras^LA2/Mmp10^−/−</i> mice were categorized as advanced adenomatous hyperplasia (AAH), or grade 1,2 or 3 adenomas using the published scoring criteria described by Jackson et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026439#pone.0026439-Jackson1" target="_blank">[28]</a>. Results are presented as the percentage of total tumors of each grade. Statistical analysis using Mann-Whitney U test revealed a significant decrease in higher grade tumors in <i>Kras^LA2/Mmp10^−/−</i> mice; *p<0.002.