Modulation of Ca²⁺ sparks with TT loss during culture.

<p>A. Representative confocal images of TT staining with di-8-ANEPPS (left) and RyR staining (right) after 48 hours of culture. B. Representative line scan image of Ca²⁺ spark protocol in CULT. The type of release areas is marked in blue and red for early and delayed release areas respectively. C. Comparison of spark duration in early (left) and delayed (right) release areas in CTRL (N_pigs = 7, n_cells = 29) vs. CULT (N_pigs = 7, n_cells = 14). D. Fraction of cells showing long-lasting sparks (>47.6 ms) in CTRL (17/35 cells with long sparks) and CULT (10/15 cells with long sparks). E. NCX current density in CTRL (N_pigs = 4, n_cells = 14) and CULT (N_pigs = 4, n_cells = 7). * denotes P<0.05.