Role of COX-2 up-regulation and of PGE₂ on platelet-dependent monocytic CD16 expression and MPA formation.

<p>(<b>A</b>) mRNA level of EP and TP receptors in freshly isolated monocytes (lane 1), and after 48 h culture either with medium alone (lane 2) or platelets (lane 3). 18S ribosomal RNA expression is shown as housekeeping RNA. Experiment is representative of n = 3. Effects of AH6809 and AH3848, alone or in combination (COMB), NS-398, aspirin or anti-PSGL-1 blocking antibody on CD16 expression (<b>B</b>) and MPA (<b>C</b>) in monocytes co-cultured with platelets for 48 h, expressed as % change in CD14⁺CD16⁺ and CD14⁺CD42b⁺ as compared to monocytes co-cultured with platelets alone. * p<0.05 <i>vs</i> M+P.