BITC inhibits angiogenesis <i>ex vivo</i>.

<p><b>A</b>. BITC inhibits VEGF-induced vessel sprouting <i>ex vivo</i>. Aortic rings (1 mm) were harvested from Sprague-Dawley rats, immerged in matrigel, and treated with VEGF (20 ng/mL) in the absence or presence of BITC (0, 2.5 and 5 and 10 µM) for 4 days and photographed under microscope (4X). Representative photographs are presented. <b>B</b>. Quantitative analysis of aortic ring assay. Aortic ring sprouting was quantified by Image J software and presented as mean ± SD of triplicates. <b>C</b>. Inhibition of CAM angiogenesis by BITC. Eggs were incubated at 37°C for 3 days. A Whatman filter disc containing the test compound (BITC 5 µmol) was placed on the CAM of eggs (n = 10) through pre-opened window and further incubated. On day 9–12 of incubation, photographs were made after removing the filter discs. A representative photograph is presented. <b>D</b>. Blood vessels density was quantified by Image J software and represented as a bar diagram.