Cathepsin expression and gross phenotype of Ctsh-deficient mice.

<p>(<b>A</b>) Western blots for Ctsh detection in lungs and liver of <i>Ctsh^+/+</i> and <i>Ctsh</i>^−/− mice. (<b>B</b>) Detection of “acidic” aminopeptidase activity at pH 6.0 in lungs, livers and kidneys of f <i>Ctsh^+/+</i> and <i>Ctsh</i>^−/− mice (n = 3). (<b>C</b>) Observed and expected genotype frequencies of litters from <i>Ctsh</i>^+/− x <i>Ctsh</i>^+/− matings. (<b>D</b>) Weight gain of female littermates from heterozygous matings (n = 5 per genotype). (<b>E</b>) mRNA expression of cathepsin C (Ctsc) and cathepsin E (Ctse) measured by quantitative ‘real-time’ RT-PCR in lungs of <i>Ctsh^+/+</i> and <i>Ctsh</i>^−/− mice (n = 5 per group). (<b>F</b>) Cathepsin C (Ctsc) and cathepsin E (Ctse) detected by Western blotting in <i>Ctsh^+/+</i> and <i>Ctsh</i>^−/− lung lysates.