Distribution of NS4 in BTV-8 and GIV infected BHK-21 cells at 24 hours post-infection.

<p><b>A:</b> BHK-21 cells infected with BTV-8 showing NS4 both in the cytoplasm and nucleus. <b>B:</b> BHK-21 cells infected with GIV showing NS4 both in the cytoplasm and nucleus. Cells were incubated with anti-BTV-8 NS4, or anti-GIV NS4 rabbit antibodies and anti-alpha tubulin mouse antibodies. Cells were then incubated with Alexa Fluor 488 (green fluorescence) conjugated anti-rabbit IgG and Alexa Fluor 568 (red fluorescence) conjugated anti-mouse.