HF treatment induces apoptosis in primary AML cells.

<p>AML cells are characterized by the French American British (FAB) M0/undifferentiated, M1/myeloblastic, M2/myeloblastic with maturation, M3/promyelocytic, M4/myelomonocytic and M5/monoblastic phenotypes. PBMCs (10⁶/ml) from normal donors (n = 4) and AML patients were cultured with or without HF 2 µg/ml for 72 h, and then stained with annexin-V-FITC/PI and analyzed by flow cytometry to assess the percentage of apoptotic cells (L2+L4 gates) in untreated (basal apoptosis) and HF-treated cultures.