Reduced size HAMLET has comparable properties to complex derived from full-length protein.

<p>αD-hLA in complex with OA has biological properties that are similar to mg-hLA OA complex with cancer cell lines SK-BR-3 and MDA-MD-468 (data not shown). αD-hLA and αD-hLA-ext, complexed with OA have comparable tumoricidal activities measured by Trypan Blue (A) and by FACS analysis using Annexin V (B), indicating that the β-domain of hLA is not required for the complex to acquire cytotoxic properties. SK-BR-3 cells were incubated with Alexa Fluor 488 labeled αD-hLA-ext either alone (C) or as OA complex (D) for 3 h. The cells were then fixed with 4% PFA in PBS, and the nuclei were counterstained with Hoechst 33342 (blue). Only αD-hLA-ext-Alexa Fluor 488 (green signal) complexed with OA is internalized by the cells.