Progress curves analysis of the complemented fluorescence.

<p><b>A. Progress curves of complemented fluorescence using purified protein.</b> The increasing fluorescence over time was determined (a.u arbitrary units) for the complementation of scFv-D1.3-GFP11 samples at different concentrations (300, 150, 75 pmol) incubated with an excess of GFP1-10 (800 pmol). Fluorescence (λ_exc = 488 nm/λ_em = 530 nm) was monitored at 3 minutes intervals for 20 h at RT. <b>B. Progress curves of complemented fluorescence using serial dilutions of crude bacteria extract.</b> A similar trend of increasing fluorescence was measured (a.u arbitrary units) directly using serial dilution of crude bacterial culture extract, albeit at lower fluorescent values. Fluorescence (λ_exc = 488 nm/λ_em = 530 nm) was monitored at 3 minutes intervals for 10 hours at RT.