Kinetics of EBV DNA and expression patterns of EBV-related genes in human tonsil tissue explants infected with B95–8.

<p>Culture medium was changed every 3 days, and collected medium was centrifuged. Cell-free supernatants and cells collected from culture medium were used for quantification of EBV DNA by real-time PCR assay and quantification of viral mRNA by real-time RT-PCR assay. Data are presented as mean ± standard error of the mean. <b>A</b>) Tissue blocks on top of collagen sponge gels in a six-well plate. <b>B</b>) Kinetics of EBV DNA in cell-free supernatants. Average data were obtained from tissues derived from 12 donors. <b>C</b>) Plots of accumulated EBV DNA in cell-free culture medium after day 12 post-infection (<i>n</i> = 12). <b>D</b>) Kinetics of EBV DNA in cells from medium (<i>n</i> = 12). <b>E</b>) Levels of EBV-related gene expressions in cells at 3, 12, and 24 days post-infection (<i>n</i> = 4).