Monosaccharide composition analysis of GAGs release by NHEKs.

<p>Media were harvested after 24 hours of culture (A) without or (B) with C-Xyloside (1 mM) stimulation and monosaccharide compositions were analyzed by HPAEC chromatograph as described under ‘<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025480#s4" target="_blank">materials and methods</a>’. The peak at 17 min may represent a monosaccharide such as glucose in the media (Glc). Arrows indicate the retention time for galactosamine (GalNH2), glucuronic acid (GlcA), and iduronic acid (IdoA). PAD: pulsed amperometric detection; nC: nano-coulomb. Data are representative of three individual experiments.