Effects of NaHS and NAC on CoCl₂-induced overproduction of reactive oxygen species (ROS) in PC12 cells.

<p>(A) Random micrographs of dichlorofluorescein (DCF)-derived fluorescence in PC12 cells. A-a: Control, untreated cells; A-b: NaHS group, cells were treated with 400 µmol/L NaHS for 30 min alone; A-c: NAC group, cells were treated with 500 µmol/L NAC for 60 min alone; A-d: CoCl₂-treated group, CoCl₂ cells treated with 600 µmol/L CoCl₂ for 6 h; A-e: NaHS+CoCl₂ group, cells were preconditioned with 400 µmol/L NaHS for 30min prior to treatment with 600 µmol/L CoCl₂ for 6 h; A-f: NAC+CoCl₂ group, cells were preconditioned with 500 µmol/L NAC for 60 min prior to treatment with 600 µmol/L CoCl₂ for 6 h; (B) Quantitative analysis of the mean fluorescence intensity in the indicated groups. Data are the mean ± SE (n = 3).^##<i>P</i><0.01 compared with control, **<i>P</i><0.01 compared with CoCl₂-treated group.