Western blot analysis of GFP protein in <i>ago6</i> mutants.

<p>Accumulation of GFP protein was observed in all four <i>ago6</i> mutants as well as <i>nrpe1-10</i> (defective in the largest subunit of Pol V), demonstrating release of <i>GFP</i> silencing. Control lanes include non-transgenic wild type plants (WT) and transgenic plants containing only the target locus (T) or the target and silencer loci (T+S). All mutants are in a T+S background. Levels of a constitutively expressed tubulin protein are shown as a loading control.