Regulation of endogenous p42.3 expression by miR-29a.

<p><b>A.</b> Effect of miR-29a mimics on the endogenous expression of p42.3 mRNA. Mature miR-29a was exogenously upregulated while the endogenous expression of p42.3 mRNA was significantly downregulated in MKN-45 cells. **: <i>P</i><0.01. <b>B.</b> Effect of miR-29a inhibitors on the endogenous expression of p42.3 mRNA. Mature miR-29a was exogenously downregulated, while the endogenous expression of p42.3 mRNA was significantly upregulated in MKN-45 cells. *: <i>P</i><0.05, **: <i>P</i><0.01. <b>C and D.</b> Effect of miR-29a mimics on the endogenous expression of p42.3, cyclin B1 and CHK2 proteins were similar to those of si-p42.3. The expression levels of p42.3 and cyclin B1 protein decreased significantly after MKN-45 cells were treated with si-p42.3 or miR-29a mimics while the level of CHK2 protein increased dramatically. Data are shown as mean ± SD (normalized; D). *: <i>P</i><0.05. <b>E and F.</b> Effect of miR-29a inhibitors on the endogenous expression of p42.3, cyclin B1 and CHK2 protein. MiR-29a inhibitors reversed the expression changes seen in C and D. *: <i>P</i><0.05, **: <i>P</i><0.01.