uPAR is not involved in the transcription of pro-inflammatory cytokines upon TLR2 stimulation in neutrophils.

<p>(A) 1×10⁶ WT and uPAR-/- neutrophils were treated with 1 µg/ml PAM for 0, 2, or 4 hours. The cells were collected and total RNA from the cells purified. The mRNA levels of TNF-α and IL-1β in the cells were determined by real-time PCR. n = 3 for each condition; Mean ± SD; All p values were greater than 0.05. (B) 1×10⁶ WT and uPAR-/- neutrophils were treated with 1 µg/ml PAM for 0, 45, or 90 min. The mRNA levels of TNF-α, IL-1β, and IL-6 in the cells were determined by real-time PCR. n = 3 for each condition; Mean ± SD; All p values were greater than 0.05. (C) 1×10⁶ WT and uPAR-/- neutrophils were treated with 1 µg/ml PAM for 4 hours. The supernatants and the neutrophils were collected and cellular extracts prepared. The supernatants and the cellular extracts were mixed and the levels of TNF-α in the mixture were determined by ELISA assays. n = 3 for each condition; Mean ± SD. Results from two independent experiments are shown.