SAV3 structural cassette expression, using recombinant baculoviruses.

<p>A) Schematic representation of the SAV3 structural cassette as it is expressed by recombinant baculoviruses. The molecular mass of the proteins are indicated and shaded areas represent transmembrane domains or signal sequences (ss). Autocatalytic (A), furin (F) and signalase (S) cleavage sites are indicated, asterisks represent N-linked glycosylation sites. B) Protein expression in <i>Sf</i>9 cells was analyzed by CBB and WB using SAV α-E1 and SAV α-E2 mabs. C) Whole cell lysates were treated with/without PNGase F and analyzed with SAV α-E2 mabs. D) Cells infected with Ac-GFP and Ac-SAV3 and stained with Hoechst. CPE was evaluated by brightfield and fluorescence microscopy. Arrows indicate dense nuclear bodies in Ac-SAV3-infected insect cells.