Coculture of FLFCs and PCMDS: Leptin production and OB-R expression.

<p><b>a</b> leptin production assessed by enzyme-linked immunosorbent assay in direct contact (co) and transwell (trw) coculture of PCMDS with FLFCs or HS-5 stromal cell lines after one week (<i>black columns</i> no contact; <i>hatched columns</i> direct end transwell contact). ***<i>P</i><0.001 versus PCMDS in control condition (Student <i>t</i> test). <b>b</b> after 48h, leptin receptor (OBR) expression was analysed by flow cytometry after immunostaining of PCMDS cultured in control conditions (<i>black columns</i>) or in co-culture with FLFCs or HS-5 (<i>hatched columns</i>).*<i>P</i><0.05 versus PCMDS in control condition (Student <i>t</i> test). <i>Columns</i>, mean of at least three independent determinations, <i>bars</i>. SD.