PCMDS, K562 and Jurkat cells cultured with stearic, oleic, linoleic and α-linolenic acids.

<p><b>a</b> lipid accumulation in PCMDS, K562 and Jurkat cell in contact with saturated (SFA) and polyunsaturated (PUFA) fatty acids (magnification, x20 and x40) → shows intracytoplasmic lipid droplets. <b>b</b> effects of α-linolenic acid on PCMDS, K562 and Jurkat cells proliferation after 48 hours. *<i>P</i><0.05 and ***<i>P</i><0.001 versus in control condition (Student <i>t</i> test). <b>c</b> effects of α-linolenic acid on PCMDS, K562 and Jurkat cells survival after 48 hours. **<i>P</i><0.01 and ***<i>P</i><0.001 versus in control condition (Student <i>t</i> test). <b>d</b> effects of 200 µM of α-linolenic, linoleic, oleic and stearic acids on Bcl-2 expression (<i>black columns</i> no lipids stimulation; <i>hatched columns</i> lipids stimulation) measured by western blot. *<i>P</i><0.05 versus PCMDS in control condition (Student <i>t</i> test). <b>e</b> effects of 200 µM of α-linolenic, linoleic, oleic and stearic acids after 24 hours on OB-R (<i>black columns)</i> no lipids stimulation; <i>hatched columns</i> lipids stimulation) and on OB-Ra (<i>black columns</i>) and OB-Rb (<i>hatched columns</i>) mRNA expression in PCMDS. *<i>P</i><0.05 and ***<i>P</i><0.001 versus in control condition <b>(</b>Student <i>t</i> test) <i>Columns</i>, mean of at least three independent determinations, <i>bars</i>. SD.