GHR levels increase in the absence of SOCS2.

<p>(A) HEK293T cells were transfected with GHR and either SOCS2 or Control siRNA. Cells were starved for 4 hours and then treated with GH for the times indicated in the picture. Cells were subsequently lysed as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025358#s4" target="_blank">Materials and Methods</a> and lysates were visualized by western blot. GHR was detected with an anti-GHR antibody. (B) GHR-levels in SOCS2 KO mice. Liver lysates from SOCS2 KO mice and control mice were visualized by western blot and analyzed for GHR levels with an anti-GHR antibody.