Distribution of NSSR1 in the acrosome of sperms from different regions of mouse epididymis.

<p>A. Representative images showing the distribution of NSSR1 (red) in sperms from different regions of mouse epididymis with nuclear stain (cyan). B. Magnified view of the sperm head in A. In the lowest lane of B, the thin gray curves merged with NSSR1 immunostaining (red) are the outlines of sperm heads. Bars = 10 µm. C. Western blot analysis of NSSR1 in sperms from different regions of mouse epididymis. Proteins from equal number of sperms were uploaded. β-actin was used as the loading control. Int: Initial region; Cap: Caput region; Corp: Corpus region; Caud: Cauda region.