Cytokine profile during liver regeneration in WT mice and in IL-1ra ko mice after hepatectomy.

<p>IL-1ra, IL-1β, TNFα and HGF secretion were analyzed by ELISA. IL-6, MCP-1 were measured by a cytometric bead array mouse inflammation kit. These cytokines were measured on peripheral blood of WT mice and IL-1ra KO mice at 4 h, 24 h, 48 h, 72h, 5 days and 7 days after partial hepatectomy. A) The level of IL-1ra in WT mice showed a significant increase between 4 h and 48 h. As expected, in IL-1ra KO mice, IL-1ra was not detectable; B-D) the levels of IL-1β, IL-6 and MCP-1 were significantly higher in IL-1ra KO mice than in WT mice with a significant difference at 4 h post-hepatectomy; E-F) there were no differences in the levels of TNFα and HGF between the two groups of mice; * = Statistical significance p<0.05. D = day IL-1ra = Interleukin-1 receptor antagonist, IL-1β = Interleukin-1β, IL-6 = Interleukin-6, MCP-1 = monocyte chemotactic protein-1, TNFα = Tumor necrosis factor- α, HGF = Hepatocyte growth factor, WT = wild type, KO = knock-out.