Blockade of PKCγ and PKC/Mζ with isozyme-specific pseudosubstrate inhibitors abolishes prenatal cocaine exposure induced increases in activated monomeric G proteins.

<p>(<b>a</b>) Organotypic FCX slice cultures were serum-depleted and treated with combination of myristoylated PKCγ- and PKC/Mζ-specific pseudosubstrate inhibitors (GIP and ZIP), 10 µM each or 10 µM control peptide, GIP/ZIP scrambled [GIP/ZIP SC] for 4 hr. Following homogenization and solubilization of the slices, GTP-bound RhoA, Rac1/Cdc42 and Rap1 as well as β-actin in the resultant lysate were either isolated by a GST fusion protein pull down method or immunoprecipitated with anti-actin. Their levels were then determined by Western blotting using specific antibodies. (<b>b</b>) Densitometric quantification of blots. <i>n</i> = 4. Data are means ± s.e.m. of the optical intensity. *p<0.01, **p<0.05 compared to respective protein in the respective GIP/ZIP SC-treated group. #p<0.01 compared to respective protein in the saline-treated group.