Enhancement of BBB permeability induced by MCP-1 improves protection in mice treated with UV-inactivated rRABV.

<p>Mice were treated with medium or MCP-1 (25 µg) by ic, BBB permeability was determined by uptake of sodium fluorescein at day 2 post treatment (A). ICR mice (group of 10) at age of 4–6 weeks were infected i.m. with 10 IMLD₅₀ DRV, and then treated with medium, MCP-1 (25 µg), 10⁷ FFU LBNSE-GM-CSF, UV-inactivated LBNSE-GM-CSF (equivalent to 10⁷ FFU) or a mixture of MCP-1 (25 µg) and UV-inactivated LBNSE-GM-CSF (equivalent to 10⁷ FFU) by ic at day 4 post infection. The mice were observed for 20 days post infection, and survivorship was recorded and analyzed. Asterisk indicates significant differences between the indicated experimental groups as calculated by one-way ANOVA or Log-rank test: <i>*, p<0.05; **, p<0.01.</i>