Effects of FOXO transcription factors on resveratrol induced apoptosis.

<p>(A and B), Phosphorylation deficient mutants of FOXO enhance resveratrol-induced apoptosis in pancreatic cancer cells. PANC-1 and AsPC-1 cells were transiently transfected with empty vector or plasmids encoding FOXO1-TM, FOXO3a-TM, or FOXO4-TM for 24 h. After transfection, culture medium was replaced with complete RPMI medium and treated with resveratrol (10 µM) for 48 h. Apoptosis was measured by TUNEL assay. Data represent the mean ± S.D. *, #, **, = significantly different from control, P<0.05. (C and D), PANC-1 and MIA PaCa-2 cells were stably transfected with either scrambled or FOXO1, FOXO3a and FOXO4 shRNA plasmids. Transfected cells were treated with resveratrol (10 µM) and apoptosis was measured by TUNEL assay. Data represent the mean ± S.D. *, # = significantly different from respective controls, P<0.05.