Effect of CORM-2 on synoviocyte proliferation and migration rate.

<p>Cells were stimulated with IL-1β (10 ng/ml) for 24 h in the presence or absence of CORM-2 (50, 100, 200 µM) or RuCl₃ (200 µM). (A) Cell proliferation was determined by the MTT assay. (B) Transwell chambers were kept in culture for 24 h. Upper compartment was detached and cells migrated to the lower side were counted in 6–8 microscopic power fields. Data (A) are expressed as mean±S.E.M. of % proliferating cells, considering 100% the proliferation induced in basal conditions whereas data (B) are expressed as % of cells migrated to the lower compartment, considering 100% the migration induced by IL-1β. Duplicate samples from 6 (A) and 4 (B) patients were used. +<i>P</i><0.05, ++<i>P</i><0.01 with respect to nonstimulated cells. **<i>P</i><0.01 with respect to IL-1β.