Transcriptional activity and relative expression of Hoxa1 variants.

<p>(A) The Hoxa1 target reporter <i>EphA2-r42B-luc</i> is activated in MCF7 cells in the presence of expression vectors for Hoxa1^WT, Hoxa1^I-V while not in the presence of Hoxa1^WM-AA or of an empty (CTL) plasmid. In each experiment, the pML-EphA2-r42B-luc reporter plasmid was transfected in combination with expression vectors for both Prep1 and Pbx1a. Results were calculated by a luciferase/β-galactosidase ratio and represented as means ± S.D. of triplicates. ***, p<0.001 (ANOVA2). (B) Detection of Hoxa1 variant proteins from whole cell lysates obtained from transiently transfected MCF7 cells reveal that Hoxa1^WT, Hoxa1^I-V and Hoxa1^WM-AA proteins are equally expressed and stable. No Hoxa1 protein could be detected from MCF7 cells or from cells transfected with an empty (CTL) expression vector. Detection of constitutively expressed β-actin protein was performed as control load.