Glutamates released from synaptic vesicles appear constancy.

<p><b>A</b>) Left panel shows a diagram for a pair-recording of uEPSCs at unitary synapses from a pyramidal to GABAergic neurons, which have a low release probability. Right panel shows the superimposed traces of uEPSCs (top) evoked by single spikes (bottom) at a synapse. <b>B</b>) The distribution of uEPSCs (non-failure portion of trails) appears single peak at this synapse, which is fitted based on Gaussian function.<b>C</b>) shows the distributions of uEPSCs without failure portion from other synapses (n = 10). <b>D</b>) shows the superimposed traces of uEPSC1, 3 and 5 induced by sequential spikes at a synapse. <b>E</b>) The distributions of uEPSC1, 3 and 5 without failure portion appear a single peak at this synapse. <b>F</b>) shows a plot of uEPSC1∼5 amplitudes averaged from other synapses (n = 7, p>0.05 for the comparison of these uEPSCs).