Influence of arginine and amino modifications at the proximal end of the 5′-duplex, on polymerization.

<p>Shown is an autoradiogram of PAGE separated polymerization products. For each sample, the polymerization products at six incubation times are shown. The number of nucleotides added to the primer during polymerization is indicated. The eighth nucleotide addition results in two bands due to nucleotide misincorporation. The length of the 5′-duplex was 17 base pairs. No difference in polymerization efficiency was found between unmodified and modified ribozymes, within the errors of three replications of the experiments.