Extension of primers that were base paired to the 5′-terminus of the polymerase ribozyme.

<p>The extension efficiency of these primers was measured as a function of the length of the 5′-duplex. (<b>A</b>) Autoradiogram of PAGE separated polymerization products, in the absence of the P2 oligo. For each length of the 5′-duplex (indicated) the unreacted primer and the reaction products are shown. (<b>B</b>) Quantitation of polymerization efficiencies. The polymerization efficiency was measured as the average number of nucleotides added per primer and plotted as a function of the length of the 5′-duplex. The polymerization efficiencies in the absence (open squares) and in the presence of the P2 oligo (filled squares) are shown. Errors are standard deviations from triplicate experiments and were usually smaller than the symbols.