MC distribution in the mouse RCAS-<i>KRas</i>+RCAS-<i>Akt</i> induced brain tumors.

<p>(<b>A</b>) Immunofluorescence staining for endothelial cell marker CD31 and MC tryptase mMCP-6 in <i>Ntv-a Arf−/−</i> and <i>Gtv-a Arf−/−</i> mouse brain tumors revealed perivascular localization of MCs. Lower panel: quantification of perivascular MCs in mouse brain tumors revealed about 50% in the corresponding objective fields with no difference between <i>Ntv-a Arf−/−</i> (n = 5) and <i>Gtv-a Arf−/−</i> (n = 5). Error bars show SD. Scale bar = 50 µM. (<b>B</b>) Immunohistochemical analysis for SCF expression revealing marked expression of SCF in the glioma vascular structures in both <i>Ntv-a Arf−/−</i> and <i>Gtv-a Arf−/−</i> mice (indicated by blue arrows). Expression of SCF was also observed in MC granules (indicated by red arrows). Lower panel: quantification of total absolute intensity signal for SCF revealed statistically significant difference between tumor and nontumor areas of the objective fields in both <i>Ntv-a Arf−/−</i> and <i>Gtv-a Arf−/−</i> mouse brain tumors (* p<0.05). Error bars show SD. Scale bar = 50 µM.