Conserved motifs and the active site of Pns6 ATPase.

<p>(A) Thin layer chromatography shows that M1 and the mutants containing M3 (M3, M23 and M13) have the strongest ATPase activity. The GKS mutant shows weaker ATPase activity than Pns6, while M2 and M12 show very little activity. BSA and GST were used as negative controls. Relative values of released ADP are presented below each lane. (B) Colorimetric malachite green assay showing the ATPase activities of the mutant proteins. All proteins were used at a range of 10 ng to 60 ng. Error bars represent standard deviations of three biological replicates.