Lentiviral infection of the wounded skin.

<p>Six mice were randomly assigned to EGF group, RhoAL63 group and RhoAN19 group (n = 2). After wound creation, 0.2 ml empty vectors (Plenti6/v5-EGFP), RhoAL63 and RhoAN19 with a titer of 1×10⁶ IU/ml were applied to the wound surface. The green fluorescence was visualized by <i>in vivo</i> fluorescence imaging system three days after infection (A, B, C), dotted line mean wound. (A′, B′, C′) The green fluorescence on fixed tissue sections was also observed under fluorescent microscope. HF, hair follicle; EP, epithelium; DE, dermis. White arrow indicates epithelium and red arrow indicates the hair follicle. (A″, B″, C″) Sections were double stained with Hoechst 33258 to visualize the nucleus. Scale bars: 100 µm.