11β-HSD1 expression is increased with starvation and differentiation.

<p>(a) The relative expression of 11β-HSD1 in NHEKs assessed by rtPCR with or without pituitary extract (pit) in culture media. GAPDH was used as an internal control. (b)Western blotting for detecting 11β-HSD1, Keratin 1, and Involucrin 48 h after adding 1.2 mM of calcium to culture media of NHEKs. The numbers indicate the relative ratio to β-actin. (c) The relative expressions of 11β-HSD1, Keratin 10, and Involucrin of the indicated hour after adding 1.2 mM calcium to culture media of NHEKs assessed by rtPCR. GAPDH was used as an internal control. An asterisk indicates a statistically significant difference (*P<0.05, **P<0.01, Student's <i>t</i>-test).