Selective inhibitor of 11β-HSD1 proliferates keratinocytes in murine skin.

<p>(a, d) Immunohistochemical staining of 11β-HSD1 (DAB staining) in C57BL/6 mouse (a) and Hos: HR-1 (hairless) mouse (d) skin tissue. Bar = 50 µM. (b, e) Immunofluorescent staining of 11β-HSD1 (red) and keratin 14 (green) in C57BL/6 mouse (b) and Hos: HR-1 mouse (e) skin tissue. Bar = 100 µM. (c, f) PCR detecting 11β-HSD1 in primary mouse keratinocytes and primary mouse dermal fibroblasts of C57BL/6 mouse (c) and Hos: HR-1 mouse (f). RT-: samples without reverse transcriptase (negative control). (g–j) Representative H&E staining (g) and Ki-67 staining (i) of 11β-HSD1 selective inhibitor or vehicle (1∶1, acetone∶olive oil) treated skin of Hos: HR-1 mice. Bar = 100 µm. (h) Epidermal thickness of vehicle and inhibitor treated mice. Intrafollicular epidermal thickness was calculated by averaging five locations in each section. Three sections from each mouse were evaluated. Bars show mean epidermal thickness ± SD of vehicle-treated mice (n = 5) and inhibitor-treated mice (n = 5; <i>*P</i><0.01, Student's <i>t</i>-test). (j) The percentage of Ki-67 positive cells. Analyses were performed by counting the total number of basal cells and cells expressing nuclear Ki-67 stain. Three sections from each mouse were evaluated. Bars indicate mean ± SD of vehicle-treated mice (n = 5) and inhibitor-treated mice (n = 5; <i>*P</i><0.05, Student's <i>t</i>-test).