Megalin, sortilin, and M6PR are expressed in cultured podocytes.

<p>(A) Immunofluorescent demonstration of megalin, sortilin, and M6PR in cultured human podocytes and (B) specific podocyte-markers podocin and nephrin. Cells were fixed, stained with the appropriate antibodies and detected with Alexa-Fluor-conjugated secondary antibodies. Controls were incubated with serum and detected with same secondary antibodies (data not shown). Nuclei (blue) were stained with DAPI. Scale bars, 5 µm. White arrows indicate potential cell surface labeling of the different receptors in the human podocytes.