Rapamycin partially inhibits proliferation of T-cell lymphoma cells and promotes eIF4E S209 phosphorylation.

<p>The CTCL-derived cell lines MyLa2059 and MyLa3675 were treated in triplicates with 200 nM of rapamycin (mTORC1i) for up to 10 days and (A) labeled with BrdU for the last 4 hr of the culture and examined using the EIA plate reader (the result shows mean value of four separate experiments) or (B) lysed and analyzed by Western blotting with antibodies against the depicted phosphorylated and total proteins with detection of actin serving as the loading control.