MNK inhibition suppresses eIF4E S209 phosphorylation.

<p>A: effect of MNK1/2 inhibitor (MNKi) used at various concentrations on eIF4E S209 phosphorylation in MyLa2059 and MyLa3675 cell lines with Akt T308 phosphorylation serving as a control. B: effect of simultaneous application of MNKi (5 µM) and mTORC1i (200 nM) on phosphorylation of eIF4E S209, S6rp S235/236, and MNK T197/202 and expression of the anti-apoptotic proteins Mcl-1 and BcL-xL. C: effect of siRNA-mediated MNK1 and MNK2 depletion on eIF4E S209 phosphorylation in the presence or absence of rapamycin. D: effect of simultaneous exposure of IL-2-dependent CTCL cell line Sez-4 to MNKi and mTORC1i on phosphorylation and expression the depicted proteins. E: effect of simultaneous treatment of patient-derived, primed Sezary CTCL cells to MNKi and mTORC1i on phosphorylation and expression the depicted proteins.