<i>TCF-1^-/-</i> mice are susceptible to EAE.

<p>(A) EAE was induced by direct immunization of WT control and <i>TCF-1^-/-</i> mice with MOG_35–55, and the EAE score was determined as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0024768#s2" target="_blank">Materials and Methods</a>; n = 3 for each group; error bars indicate ±SD. (B) Reduced number of T cells in <i>TCF-1^-/-</i> mice. Numbers of peripheral CD4 cells in WT control or <i>TCF-1^-/-</i> mice; n = 4; error bars indicate ±SEM. (C) Increased Th17 cells in <i>TCF-1^-/-</i> mice. Peripheral lymphocytes were restimulated with MOG_35–55 and IL-23 after isolation from MOG_35–55-immunized WT control and <i>TCF-1^-/-</i> mice. IL-17A was measured by intracellular staining. Shown contour plots are representative of three independent experiments. (D) <i>Rag1^-/-</i> mice adoptively transferred with <i>TCF-1^-/-</i> T cells are susceptive to EAE induction. 1×10⁶ or 3×10⁶ WT CD4 cells, or 1×10⁶<i>TCF-1^-/-</i> CD4 cells were transferred into <i>Rag1^-/-</i> mice. One day later, mice were immunized with MOG_35–55 to induce EAE; n = 6 for each group; error bars indicate ±SEM.