Dose-dependent <i>NELL-1</i> expression response to Osterix.

<p>(<b>A</b>) Human <i>NELL-1</i> promoter is responsive to Osterix in a dose-dependent manner shown by reporter assay. Graph detailing the luciferase activity in Saos-2 cells co-transfected with either the p2213WT-Luc or the p325WT-Luc promoter-luciferase constructs as well as 0, 0.25, 0.5, or 1 ug of Osterix expression vector (pOsx). Data are expressed as a percentage of the luciferase activity of the p325WT-Luc construct in the absence of control vector (pCtr). (<b>B</b>) Osterix overexpression decreased <i>NELL-1</i> mRNA level in Saos-2 cells at 2 days and 7 days post-transfection. Western blot showed Osterix protein levels at 2 days and 7 days post-transfection. (*p<0.05) (<b>C</b>) Osterix transcriptional repression of NELL-1 promoter in osteoblastic and non-osteoblastic cells. A graph detailing the luciferase activity of p325WT-Luc 2 days post-transfection in Saos-2, U2OS, Hela and Glioma cell lines co-transfected with pCtr or pOsx. (*p<0.05)