Differential effect of gemfibrozil in RLIP76^{<b>+<b>/</b>+</b>} and RLIP76^−/− mice.

<p><b><u>Panel A</u>:</b> Effect of RLIP76 depletion by RLIP76 antisense on triglycerides level in RLIP76^+/+ mice. p<0.02, when compared to scrambled antisense treatment. <b><u>Panel B</u>:</b> triglycerides level was measured prior to and 24 h after a single oral dose of gemfibrozil (100 mg/kg b.w.) by gavage in RLIP76^+/+ and RLIP76^−/− mice. p<0.001, when compared between RLIP76^+/+ and RLIP76^−/− mice, and p<0.02 when compared to gemfibrozil treatment in RLIP76^+/+ mice. In panels A & B, 5 mice per group were used. <b><u>Panel C</u>:</b> Effect of gemfibrozil on PPARα expression by Western blot in mouse liver tissue lysates, and developed bands were quantified by scanning densitometry. GAPDH expression was used as loading control. WT, wild-type; KO, RLIP76-knockout; Gemf, gemfibrozil; <b><u>Panel D</u>:</b> Effect of gemfibrozil on PPARα expression in paraffin embedded RLIP76^+/+ and RLIP76^−/− mouse liver tissues section by immuno-histochemistry using ABC staining kit (Vector). Immuno-reactivity is evident as a dark brown stain, whereas non-reactive areas display only the background color. Sections were counter-stained with Hematoxylin (blue). Photographs at 40× magnification were acquired using Olympus Provis AX70 microscope. Percent staining was determined by measuring positive immuno-reactivity per unit area. Arrows represent the area for positive staining for an antigen. The intensity of antigen staining was quantified by digital image analysis. Bars represent mean ± S.E. (n = 5); * p<0.002 compared with control.