TMZ-resistant cells show a higher mitochondrial coupling.

<p>Oxygen consumption rates of isolated mitochondria (200 µg) were determined using a respirometer. Graphic illustrations of sample traces of mitochondrial respiration rates of (A) U251 and (B) UTMZ cells (dotted line, oxygen concentration; solid line, oxygen consumption). (C) Kinetic characterization of glutamate/malate-dependent respiration of isolated mitochondria from U251 and UTMZ cells. (D) Kinetic characterization of glutamate/malate-dependent respiration of isolated mitochondria from TMZ-sensitive and resistant GBM xenolines. Data are expressed as means ± S.E. of three independent experiments. <i>p</i><0.05 (*), <i>p</i><0.01 (**) and <i>p</i><0.001 (***).