The density of KCC2 is constant along dendrites of neurons in culture.

<p><b>A</b>. Three confocal images tiled showing a neuron immunostained with a fluorescent anti-KCC2 antibody Images are of size 1024×1024pixels with a pixel size of 0.115 µm and a 9.1 µs pixel dwell time. Three red squares representing example regions analyzed are shown, in <b>B</b>, with their corresponding binary mask used to delineate the labeled dendrite. The intensity histograms of the representative subregions delimited in <b>A</b> are shown in <b>B</b> with their corresponding SpIDA fit and recovered values of monomer (N₁) and dimer (N₂) densities. The distance (e.g., <i>d</i> in <b>A</b>) between the center of the cell body and the center of the analyzed region was also measured. <b>C</b>. Graph of the density of KCC2 dimers at the dendrite surface as a function of the distance from the cell body obtained with SpIDA. 823 regions were analyzed from 27 neurons. Error bars show SEM.