DβHB and LβHB do not inhibit HDAC activities.

<p>Hela nuclear extract was used as a source of class I and II HDAC activity (<b>Panel A</b>) where as recombinant human Sir1 was used as a source of class III HDAC activity (<b>Panel B</b>). HDAC activities were assessed in the absence (control, “C”) or presence of 4 mM DβHB (“D”), LβHB (“L”) or sodium butyrate (“Na”, an HDAC I and II inhibitor), 1 µM trichostatin A (“TA”, an HDAC I and II inhibitor) or 1 mM nicotinamide (“Nic”, an HDAC III inhibitor). Activities were quantified based on fluorescence intensity and expressed as arbitrary fluorescence unit (AFU). <i>n</i> = 6–16 per group from 5 independent experiments.