<p>Freezed/thawed antigens of <i>L. major</i> were used to stimulate the PBMCs in culture as previous disease indicator. The responses of <i>L. major</i> recovered individuals were potentially detected at CD4 level. Each point represents response of each individual. Horizontal bars represent the median value of CD4<sup>+</sup>/IFN γ<sup>+</sup> T cells in the related group. Statistical analysis shows a significant difference between R.A2<sup>+</sup> (HLA-A2<sup>+</sup> recovered individuals) and R.A2<sup>−</sup> (HLA-A2<sup>−</sup> recovered individuals) groups with H.A2<sup>+</sup> individuals (HLA-A2<sup>+</sup> healthy donors) with <i>p</i> value<0.05.</p
<p>PBMCs were stimulated with anti-CD3 for 18 h or media alone. Cells were surface stained with anti...
<p>(A and B) Fresh CD8-depleted PBMCs from 12 adult individuals with documented primary HIV infectio...
<p>T-cell responses determined by IFN-γ ELISpot from donors treated with mAb1, un, sl1 and sl2 mater...
<p>Percentages of CD4<sup>+</sup> cells bearing activation markers: CD4<sup>+</sup>CD69<sup>+</sup> ...
<p>IFN-γ production was measured in culture supernatants of HLA-A2<sup>+</sup> recovered individuals...
<p>(A) Schematic of experimental procedure. Primary CD4<sup><b>+</b></sup> T cells were isolated fro...
<p>6 out of 19 (31.6%) and 2 out of 15 (13.3%) HLA-A2<sup>+</sup> recovered individuals responded ab...
<p>The distribution of CD3<sup>+</sup> cells, CD4<sup>+</sup> cells, CD8<sup>+</sup> cells(A), B cel...
<p>Cytokine concentrations were measured in cell culture supernatants using multiplex analysis. Medi...
<p>LTT response, proliferation was represented as mean O.D of stimulated culture - mean O.D of unsti...
<p>IFNγ production in SLA stimulated WB cultures of active VL patients following depletion of a) CD4...
<p>Venous blood samples from 4 healthy donors were stimulated with SEB for 24 hours either as whole ...
<p>A. Geometric mean CS-specific CD4<sup>+</sup> T cells producing IFN-γ by ELISpot. Comparisons bet...
<p>PBMC from acute patients (n = 24) were stained for intracellular cytokines and the percentage of ...
<p>Cryopreserved PBMC were thawed and analysed. Representative FACS plots indicating our gating stra...
<p>PBMCs were stimulated with anti-CD3 for 18 h or media alone. Cells were surface stained with anti...
<p>(A and B) Fresh CD8-depleted PBMCs from 12 adult individuals with documented primary HIV infectio...
<p>T-cell responses determined by IFN-γ ELISpot from donors treated with mAb1, un, sl1 and sl2 mater...
<p>Percentages of CD4<sup>+</sup> cells bearing activation markers: CD4<sup>+</sup>CD69<sup>+</sup> ...
<p>IFN-γ production was measured in culture supernatants of HLA-A2<sup>+</sup> recovered individuals...
<p>(A) Schematic of experimental procedure. Primary CD4<sup><b>+</b></sup> T cells were isolated fro...
<p>6 out of 19 (31.6%) and 2 out of 15 (13.3%) HLA-A2<sup>+</sup> recovered individuals responded ab...
<p>The distribution of CD3<sup>+</sup> cells, CD4<sup>+</sup> cells, CD8<sup>+</sup> cells(A), B cel...
<p>Cytokine concentrations were measured in cell culture supernatants using multiplex analysis. Medi...
<p>LTT response, proliferation was represented as mean O.D of stimulated culture - mean O.D of unsti...
<p>IFNγ production in SLA stimulated WB cultures of active VL patients following depletion of a) CD4...
<p>Venous blood samples from 4 healthy donors were stimulated with SEB for 24 hours either as whole ...
<p>A. Geometric mean CS-specific CD4<sup>+</sup> T cells producing IFN-γ by ELISpot. Comparisons bet...
<p>PBMC from acute patients (n = 24) were stained for intracellular cytokines and the percentage of ...
<p>Cryopreserved PBMC were thawed and analysed. Representative FACS plots indicating our gating stra...
<p>PBMCs were stimulated with anti-CD3 for 18 h or media alone. Cells were surface stained with anti...
<p>(A and B) Fresh CD8-depleted PBMCs from 12 adult individuals with documented primary HIV infectio...
<p>T-cell responses determined by IFN-γ ELISpot from donors treated with mAb1, un, sl1 and sl2 mater...