eIF2A mRNA and protein are turned over during stress conditions.

<p><i>A.</i> Schematic diagram of the YCplac111-YP plasmid containing N-terminally HA-tagged eIF2A under the control of its native promoter. This plasmid contains a <i>LEU2</i> selection cassette and is used throughout the experiments presented in this manuscript, except where otherwise denoted. <i>B,</i> mRNA analysis (upper panels) and Western blot analysis of eIF2A and <i>PGK1</i> transcripts and protein levels (lower panels) indicating eIF2A mRNA is turned over during both 6% ethanol and 1M sorbitol stress in BY4684 ΔeIF2A yeast transformed with YCplac111-YP illustrated in <i>A</i>.