Tat-mediated increase of AP and depolarization of RMP were not affected by roscovitine.

<p>Tat at 20 μM was focally applied to an individual DRG neuron to assess its actions over a 4 min time period, thereafter, the neuron was exposed to 10 μM roscovitine by external perfusion of the recording chamber. The whole-cell patch-clamp recordings were used to determine APs prior to Tat exposure (control), exposure to Tat or roscovitine or both. (A) A representative experiment of AP recording from one of five DRG neurons is shown. (B) Summary data from time points of Tat alone (n = 15) and Tat plus roscovitine co-exposure performed on all five DRG neurons are shown. Bars represent SEM on the mean. Ros: roscovitine, APs: action potentials.