ch-4C5 inhibits the metastatic deposition of MDA-MB-453 cancer cells into the lungs of SCID mice.

<p>Control or ch-4C5 treated MDA-MB-453 cells were labeled with the fluorescent dye DiO and injected into SCID mice as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0023906#s4" target="_blank">Materials and Methods</a>. Evaluation of metastatic deposits was performed several hours later. A. Representative cryosections of the lungs of control and ch-4C5 treated mice. The arrows show MDA-MB-453 cells stained with DiO present in the lung tissue. A significant decrease in the deposition of cancer cells was observed in the lungs of ch-4C5 treated mice. Scale bar: 100 µm <b>B</b>. Quantitative effect of ch-4C5 on the metastatic deposition of MDA-MB-453 cells into the lungs, showed a 38% inhibition when compared to control animals. The bars represent the average of two independent experiments ±SEM. Statistical significance of differences was tested by Student's t test (p<0.01).