Flow cytometer data illustrating the impact of IL-15 (1 ng/ml) treated NK-DC co-culture on the expression of DC maturation (HLA-DR FITC) and chemokine (CCR7 PE) markers, comparing responses for DCs with either HLA-C1 (<b>figures 6b, d, f</b>) or HLA-C2 (<b>figures 6c, e, g</b>) homozygous alleles.

<p>(a) shows DC stained with isotype control for HLA-DR & CCR7, (b) & (c) shows background DC staining where DCs are in isolation in the presence of IL-15, (d) & (e) shows HLA-DR & CCR7 expression by DC in NK-DC co-culture at ratios of 1∶1 in the presence of IL-15, (f) & (g) shows DC markers in NK-DC co-culture at ratios 1∶5 in the presence of IL-15. This data clearly demonstrates that in NK-DC co-culture at ratios of 1∶1 in the presence of IL-15, HLA-C1 homozygous DCs undergo significantly greater maturation than HLA-C2 homozygous DCs and attain CCR7 chemokine expression required for trafficking to secondary lymphoid tissues. Results are representative of 4 experiments with 5,000 DC gated events captured.