Helios expression was not restricted to CD4 cells, but among CD4+ cells correlated with Treg-associated markers.

<p>(A) Expression of Helios in murine lymphocytes (left column) and CD25+ Foxp3+ co-expression in CD4+ Helios+ gated subsets (right column) of blood (top), lymph nodes (middle) and spleen (bottom). (B) Expression of Helios in human lymphocytes (top) and co-expression of Helios with Treg-associated markers in cells gated for CD4 expression. Right column, bottom row shows absence of correlation between Helios and CD31, a marker of recent thymic emigrants cells. Data are representative of 5 experiments.