IL-17 and IFN-γ production in PBMC peaks 2 weeks post-vaccination.

<p>PBMC from vaccinated subjects were stimulated with Ag85A peptide pools with or without 100 uM ARL67156. No stimulation and phorbol 12-myristate 13-acetate with ionomycin were used as negative and positive controls. Percentages shown are unstimulated subtracted from Ag85A stimulation. Following staining, cells were gated as shown in (A): Lymphocytes were gated for on FSC vs. SSC. Singlets were then gated and dead cells, B cells and monocytes were gated out. CD3⁺ cells were selected for CD4⁺CD8⁻ cells. Antigen-specific cytokine expression from these cells was evaluated. Cells expressing both IL-17 and IFN-γ were quantified and shown in (B). The effect of ARL67156 on cytokine expression was investigated by addition during ICS stimulation (C).